Vitality, an asking need of the everyday life is continuing draining while the interest for is expanding with time biohydrogen innovation gives an elective wellspring of vitality and hails as a normal fitting innovation that meets the fundamental requirement for cooking fuel in country India. The point of the examination is to decide the reason for the structure of an Anaerobic Sequencing Batch Reactor (AnSBR) worked in mesophilic conditions for the treatment of refinery wastewater. To contemplated the qualities of crude refinery wastewater, for example, pH = 3.9, BOD = 40000 mg/l, COD = 100000 mg/l, TS = 95000 mg/l and attributes of slime pH = 5.3, TS = 107000 mg/l were likewise broke down. At first the treatment of refinery emanating by an AnSBR at lab scale was completed utilizing a reactor of 5 L volume. The reactor was structured with 10 cm breadth and 62 cm tallness, the gas get away from valve and different tests are 2 cm. The reactor was feed with two diverse sythesis of refinery wastewater and slop is kept up at about fourteen days. The extent of piece is one contain 25% of ooze and 75% of wastewater and another have 30% of muck and 70% of wastewater. The AnSBR was worked in 24h, 48h and 72h HRT with different patterns of OLR. The OLR was 2.5 kg COD/m3.d, 3.5 kg COD/m3.d and 4.5 kg COD/m3.d. The greatest Hydrogen Production Rate is achieved in 2.5kg COD/m3 at 72h HRT for stacking condition - 1 with pH = 5.3 and COD evacuation effectiveness is 69.27%.